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Invitrogen
Human CLPH (aa 101-200) Control Fragment Recombinant Protein
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Product Details
RP-100151
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GTTNSITRDSITEHFMPVKIGNISSPVTTVSLIDFSTDIAKEDILLATIDTGDAEISITSEVSGTLKDSSAGVADAPAFPRKKDEADMSNYNSSIKSNVP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (58%), Rat (58%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60590. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
CLPH (Casein-Like phosphoprotein) is a recently discovered, evolutionarily conserved, testis-specific cytoplasmic protein expressed exclusively in postmeiotic germ cells in rat spermatogenesis. CLPH is a novel casein kinase 2-phosphorylated disordered and calcium-binding protein that displays strong variation in relative expression levels during spermatogenesis. It is frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation and is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium-binding and spermatid-specific protein 1; calcium-binding protein, sperm-specific 1; calcium-binding protein, spermatid-specific 1; casein-like phosphoprotein; Testis development protein NYD-SP26
Gene Aliases: C4orf35; CABS1; CLPH; NYD-SP26
UniProt ID: (Human) Q96KC9
Entrez Gene ID: (Human) 85438
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