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Invitrogen
Human CTU2 Control Fragment Recombinant Protein
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Product Details
RP-98583
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
LSPDSFCLGFSEGAACGQSLEERSKTLAEVKPILQATGFPWHVVALEEVFSLPPSVLWCSAQELVGSEGAYKAAVDSFLQQQH
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (69%), Rat (69%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59441. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytoplasmic tRNA 2-thiolation protein 2; Cytosolic thiouridylase subunit 2; cytosolic thiouridylase subunit 2 homolog
Gene Aliases: C16orf84; CTU2; NCS2; UPF0432
UniProt ID: (Human) Q2VPK5
Entrez Gene ID: (Human) 348180
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