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Invitrogen
Human DEF6 (aa 521-610) Control Fragment Recombinant Protein
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Product Details
RP-94601
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
RADEDVEAAQRKLRQASTNVKHWNVQMNRLMHPIEPGDKRPVTSSSFSGFQPPLLAHRDSSLKRLTRWGSQGNRTPSPNSNEQQKSLNGG
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58619. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
DEF6, or IBP, is a guanine nucleotide exchange factor for RAC and CDC42 that is highly expressed in B and T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DEF-6; DEF6 guanine nucleotide exchange factor; Differentially expressed in FDCP 6 homolog; IRF4-binding protein; OTTHUMP00000016251; RP3-329A5.5; SWAP-70-like adaptor protein of T cells
Gene Aliases: DEF6; IBP; SLAT; SWAP70L
UniProt ID: (Human) Q9H4E7
Entrez Gene ID: (Human) 50619
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