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Invitrogen
Human DHCR7 (aa 356-414) Control Fragment Recombinant Protein
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Product Details
RP-91294
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
HQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMG
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (92%), Rat (92%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60666. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-dehydrocholesterol reductase; 7-DHC reductase; Cholesterol-5,6-epoxide hydrolase subunit DHCR7; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; putative sterol reductase SR-2; Sterol Delta(7)-reductase; sterol delta-7-reductase; Sterol reductase SR-2
Gene Aliases: D7SR; DHCR7; SLOS
UniProt ID: (Human) Q9UBM7
Entrez Gene ID: (Human) 1717
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