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Invitrogen
Human DOC2B (aa 235-266) Control Fragment Recombinant Protein
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Product Details
RP-100079
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
LKKLKPNHTKTFSICLEKQLPVDKTEDKSLEE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (97%), Rat (97%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60265. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
There are at least two protein isoforms of the Double C2 protein, namely alpha and beta, which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca-dependent intracellular vesicle trafficking in various types of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Doc2-beta; Double C2-like domain-containing protein beta; double C2-like domains, beta; OTTMUSP00000006522
Gene Aliases: DOC2B; DOC2BL
UniProt ID: (Human) Q14184
Entrez Gene ID: (Human) 8447
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