Invitrogen

Human EHD3 (aa 27-77) Control Fragment Recombinant Protein

Product Details

RP-100845

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

KLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRY

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-62079. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The EHD3 gene encodes the EH-domain containing protein 3, part of the EHD protein family involved in the regulation of endocytic recycling and membrane trafficking. EHD3 plays a crucial role in the endosomal transport system, impacting how receptors and other membrane proteins are recycled back to the cell surface after internalization. This protein aids in maintaining cellular homeostasis by ensuring the efficient sorting and transport of cargo through the endosomal system. EHD3 has been implicated in various cellular functions, including insulin signaling, receptor recycling, and neuronal development, and significantly influences synaptic transmission and plasticity. Alterations in EHD3 expression or function can disrupt endosomal trafficking processes and are linked to conditions such as neurodegenerative diseases and cancer. Research on EHD3 is focused on uncovering its role in membrane dynamics and identifying potential therapeutic strategies for disorders involving dysfunctional endocytic pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: EH domain-containing protein 3; EH-domain containing 3; PAST homolog 3