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Gibco
Human EPO (Erythropoietin) Recombinant Protein
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Product Details
PHC2054
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human,
Mouse
Expression System
CHO cells
Amino acid sequence
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
Molecular weight
30.4 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
Specific Activity = 120,000 IU/mg; determined by in vivo bioassay measuring the dose-dependent increase in reticulocyte count in normocythaemic mice.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
0.006mg citric acid/0.58mg sodium citrate with 0.58mg NaCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, deionized water to a concentration of 12,000-120,000 IU/mL. Further dilutions can be made in aqueous buffers. For long term storage, EPO solutions should be supplemented with carrier protein, such as 0.1% HSA or BSA.
Storage: This lyophilized preparation is stable at room temperature for three weeks, but should be stored desiccated below -18°C. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C, but should be stored below -18°C for long term storage. Avoid repeated freeze/thaw cycles.
Target Information
Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epoetin; Erythropoietin; MGC138142
Gene Aliases: EP; EPO; MVCD2
UniProt ID: (Human) P01588
Entrez Gene ID: (Human) 2056
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