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Invitrogen
Human Epsin 2 (aa 213-265) Control Fragment Recombinant Protein
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Product Details
RP-106456
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
PLGQSEELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSEL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (92%), Rat (92%).
This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Epsin2 plays a role in the formation of clathrin-coated invaginations and endocytosis. It binds to EPS15 (via the NPF repeat domain), as well as to AP-2 and clathrin (via the DPW repeat domain). The protein resides in the cytoplasm, in punctate structures throughout the cell, associated with clathrin-coated vesicles, and is particularly concentrated in the region of the Golgi complex. Highest expression is found in brain, with lower levels detected in lung and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EPS-15-interacting protein 2; Eps15 binding protein; Epsin-2
Gene Aliases: EHB21; EPN2; KIAA1065
UniProt ID: (Human) O95208
Entrez Gene ID: (Human) 22905
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