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Invitrogen
Human GAGE1 (aa 20-56) Control Fragment Recombinant Protein
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Product Details
RP-104354
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
PPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAA
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (39%), Rat (39%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60286. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs. The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen MZ2-F; Cancer/testis antigen 4.1; cancer/testis antigen family 4, member 1; CT4.1; G antigen 1; GAGE-1; MZ2-F antigen
Gene Aliases: CT4.1; GAGE-1; GAGE1
UniProt ID: (Human) P0DTW1
Entrez Gene ID: (Human) 2543
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