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Invitrogen
Human GPR31 (aa 197-222) Control Fragment Recombinant Protein
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Product Details
RP-90860
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
NAGIIRALQKRLREPEKQPKLQRAQA
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (62%), Rat (62%).
This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. GPR31 is a high-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding leads to activation of MAPK and NF-kappa-B pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12-(S)-HETE acid receptor; 12-(S)-HETE receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; bA517H2.2 (G protein-coupled receptor 31); G-protein coupled receptor 31; GPR31/12-HETER; hydroxyeicosatetraenoic (HETE) acid receptor 1; probable G-protein coupled receptor 31; TCP10C
Gene Aliases: 12-HETER; GPR31; HETER; HETER1
UniProt ID: (Human) O00270
Entrez Gene ID: (Human) 2853
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