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Invitrogen
Human GTF2A1 (aa 98-248) Control Fragment Recombinant Protein
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Product Details
RP-88980
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
QQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQA
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-81901. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A) and several general initiation factors. One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: general transcription factor IIA 1; General transcription factor IIA subunit 1; general transcription factor IIA, 1, 19/37kDa; glucose regulated protein, 58kD pseudogene; TFIIA alpha, p55, isoform 1; TFIIA alpha/beta subunits; TFIIA-42; TFIIAL; Transcription initiation factor IIA subunit 1; Transcription initiation factor TFIIA 42 kDa subunit
Gene Aliases: GTF2A1; TF2A1; TFIIA; TFIIA-42; TFIIAL
UniProt ID: (Human) P52655
Entrez Gene ID: (Human) 2957
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