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Invitrogen
Human GTF2A1L (aa 90-145) Control Fragment Recombinant Protein
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Product Details
RP-110061
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GRTLPSFTTAELGTSNSSANFTFPGYPIHVPAGVTLQTVSGHLYKVNVPIMVTETS
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (73%), Rat (73%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-145071. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CXCDC1; General transcription factor II A, 1-like factor; general transcription factor IIA 1 like; general transcription factor IIA 1-like; general transcription factor IIA, 1-like; GTF2A1-like factor; KIAA2037; testis secretory sperm-binding protein Li 230m; TFIIA large subunit isoform ALF; TFIIA-alpha and beta-like factor; TFIIA-alpha/beta-like factor
Gene Aliases: ALF; GTF2A1L; GTF2A1LF
UniProt ID: (Human) Q9UNN4
Entrez Gene ID: (Human) 11036
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