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Invitrogen
Human H2BFWT (aa 47-89) Control Fragment Recombinant Protein
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Product Details
RP-100384
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
PKEANSTTSQKQSKQRKRGRHGPRRCHSNCRGDSFATYFRRVL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (34%), Rat (34%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63280. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Atypical histone H2B. Nucleosomes containing it are structurally and dynamically indistinguishable from those containing conventional H2B. However, unlike conventional H2B, does not recruit chromosome condensation factors and does not participate in the assembly of mitotic chromosomes. May be important for telomere function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H2B histone family member W testis-specific; H2B histone family, member W, testis-specific; H2B.W histone 1; Histone H2B type W-T
Gene Aliases: H2BFWT; H2BW1; TH2B-175
UniProt ID: (Human) Q7Z2G1
Entrez Gene ID: (Human) 158983
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