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Invitrogen
Human ICAM-1 (CD54) His-tag Fc Chimera Recombinant Protein
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Product Details
A42523
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human ICAM1, amino acids Met1-Glu480 (Accession # NP_000192.2) with a C-terminal His-tagged human IgG1 Fc-tag
Tag
Fc-tag, His-tag
Molecular weight
77.4 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 µg/mL with 100 µL/well), >40% cells will adhere after 1 hour of incubation at 37°C.
When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 µg/mL with 100 µL/well), >40% cells will adhere after 1 hour of incubation at 37°C.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells
Gene Aliases: BB2; CD54; ICAM1; P3.58
UniProt ID: (Human) P05362
Entrez Gene ID: (Human) 3383
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