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Human IL-15, Animal-Free Recombinant Protein, PeproTech®

Human IL-15, Animal-Free Protein in Functional Assay (Functional)

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Human IL-15, Animal-Free Protein (AF-200-15-10UG) in Functional

Bioassay analysis of Human IL-15, Animal-Free Recombinant Protein, PeproTech® (Product # AF-200-15-1MG).

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Product Details

AF-200-15-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 49 publications 49 publications

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Hamster, Human, Mouse

Expression System

E. coli

Amino acid sequence

MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS

Molecular weight

12.9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of mouse CTLL-2 cells. The expected ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-15 is a 12.9 kDa protein consisting of 115 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade.

Cancer cell

Srivastava S,Furlan SN,Jaeger-Ruckstuhl CA,Sarvothama M,Berger C,Smythe KS,Garrison SM,Specht JM,Lee SM,Amezquita RA,Voillet V,Muhunthan V,Yechan-Gunja S,Pillai SPS,Rader C,Houghton AM,Pierce RH,Gottardo R,Maloney DG,Riddell SR

Mon Feb 08 00:00:00 UTC 2021

SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2.

Leukemia

von Wenserski L,Schultheiß C,Bolz S,Schliffke S,Simnica D,Willscher E,Gerull H,Wolters-Eisfeld G,Riecken K,Fehse B,Altfeld M,Nollau P,Binder M

Thu Apr 01 00:00:00 UTC 2021

A T cell redirection platform for co-targeting dual antigens on solid tumors.

mAbs

Enderle L,Shalaby KH,Gorelik M,Weiss A,Blazer LL,Paduch M,Cardarelli L,Kossiakoff A,Adams JJ,Sidhu SS

AF-200-15 was used in Cell Culture to engineer the Dual Antigen T cell Engager to enable T cell activity to specific solid cancer cell features.

Tue Dec 28 00:00:00 UTC 2021

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells.

Nature communications

Kluesner MG,Lahr WS,Lonetree CL,Smeester BA,Qiu X,Slipek NJ,Claudio Vázquez PN,Pitzen SP,Pomeroy EJ,Vignes MJ,Lee SC,Bingea SP,Andrew AA,Webber BR,Moriarity BS

Fri Apr 23 00:00:00 UTC 2021

A CAR RNA FISH assay to study functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue.

Scientific reports

Eichholz K,Li AZ,Diem K,Jensen MC,Zhu J,Corey L

Mon Jun 21 00:00:00 UTC 2021

Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer.

Nature communications

Weng RR,Lu HH,Lin CT,Fan CC,Lin RS,Huang TC,Lin SY,Huang YJ,Juan YH,Wu YC,Hung ZC,Liu C,Lin XH,Hsieh WC,Chiu TY,Liao JC,Chiu YL,Chen SY,Yu CJ,Tsai HC

Mon Apr 12 00:00:00 UTC 2021

Oncolytic measles virus therapy enhances tumor antigen-specific T-cell responses in patients with multiple myeloma.

Leukemia

Packiriswamy N,Upreti D,Zhou Y,Khan R,Miller A,Diaz RM,Rooney CM,Dispenzieri A,Peng KW,Russell SJ

Tue Dec 01 00:00:00 UTC 2020

TGF-β and IL-15 Synergize through MAPK Pathways to Drive the Conversion of Human NK Cells to an Innate Lymphoid Cell 1-like Phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Hawke LG,Mitchell BZ,Ormiston ML

Mon Jun 15 00:00:00 UTC 2020

Assessment of long non-coding RNA expression reveals novel mediators of the lung tumour immune response.

Scientific reports

Sage AP,Ng KW,Marshall EA,Stewart GL,Minatel BC,Enfield KSS,Martin SD,Brown CJ,Abraham N,Lam WL

Fri Oct 09 00:00:00 UTC 2020

Efficacy of anti-CD147 chimeric antigen receptors targeting hepatocellular carcinoma.

Nature communications

Tseng HC,Xiong W,Badeti S,Yang Y,Ma M,Liu T,Ramos CA,Dotti G,Fritzky L,Jiang JG,Yi Q,Guarrera J,Zong WX,Liu C,Liu D

Wed Sep 23 00:00:00 UTC 2020

Distinctive phenotypes and functions of innate lymphoid cells in human decidua during early pregnancy.

Nature communications

Huhn O,Ivarsson MA,Gardner L,Hollinshead M,Stinchcombe JC,Chen P,Shreeve N,Chazara O,Farrell LE,Theorell J,Ghadially H,Parham P,Griffiths G,Horowitz A,Moffett A,Sharkey AM,Colucci F

Mon Jan 20 00:00:00 UTC 2020

TCR-induced alteration of primary MHC peptide anchor residue.

European journal of immunology

Madura F,Rizkallah PJ,Legut M,Holland CJ,Fuller A,Bulek A,Schauenburg AJ,Trimby A,Hopkins JR,Wells SA,Godkin A,Miles JJ,Sami M,Li Y,Liddy N,Jakobsen BK,Loveridge EJ,Cole DK,Sewell AK

Mon Jul 01 00:00:00 UTC 2019

Gene correction for SCID-X1 in long-term hematopoietic stem cells.

Nature communications

Pavel-Dinu M,Wiebking V,Dejene BT,Srifa W,Mantri S,Nicolas CE,Lee C,Bao G,Kildebeck EJ,Punjya N,Sindhu C,Inlay MA,Saxena N,DeRavin SS,Malech H,Roncarolo MG,Weinberg KI,Porteus MH

Tue Apr 09 00:00:00 UTC 2019

Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants.

Nature communications

Koenig A,Chen CC,Marçais A,Barba T,Mathias V,Sicard A,Rabeyrin M,Racapé M,Duong-Van-Huyen JP,Bruneval P,Loupy A,Dussurgey S,Ducreux S,Meas-Yedid V,Olivo-Marin JC,Paidassi H,Guillemain R,Taupin JL,Callemeyn J,Morelon E,Nicoletti A,Charreau B,Dubois V,Naesens M,Walzer T,Defrance T,Thaunat O

Mon Nov 25 00:00:00 UTC 2019

NK cells are activated and primed for skin-homing during acute dengue virus infection in humans.

Nature communications

Zimmer CL,Cornillet M,Solà-Riera C,Cheung KW,Ivarsson MA,Lim MQ,Marquardt N,Leo YS,Lye DC,Klingström J,MacAry PA,Ljunggren HG,Rivino L,Björkström NK

Thu Aug 29 00:00:00 UTC 2019

Human leucocyte antigen class I-redirected anti-tumour CD4⁺ T cells require a higher T cell receptor binding affinity for optimal activity than CD8⁺ T cells.

Clinical and experimental immunology

Tan MP,Dolton GM,Gerry AB,Brewer JE,Bennett AD,Pumphrey NJ,Jakobsen BK,Sewell AK

Sun Jan 01 00:00:00 UTC 2017

In vitro Homeostatic Proliferation of Human CD8 T Cells.

Bio-protocol

Abdelsamed HA,Zebley CC,Youngblood B

AF-200-15 was used in Cell Culture to provide a protocol for the assessment of epigenetic programs in divided and undivided human naïve and memory CD8 T cells following 7 days in culture with IL-7 and IL-15 to illustrate how this approach can shed light on the mechanism(s) that governs the preservation of effector functions during homeostasis of long-lived memory CD8 T cells.

Mon Nov 20 00:00:00 UTC 2017

Phase 1 trial of IL-15 trans presentation blockade using humanized Mikβ1 mAb in patients with T-cell large granular lymphocytic leukemia.

Blood

Waldmann TA,Conlon KC,Stewart DM,Worthy TA,Janik JE,Fleisher TA,Albert PS,Figg WD,Spencer SD,Raffeld M,Decker JR,Goldman CK,Bryant BR,Petrus MN,Creekmore SP,Morris JC

Thu Jan 17 00:00:00 UTC 2013

Phase 1 trial of IL-15 trans presentation blockade using humanized Mikβ1 mAb in patients with T-cell large granular lymphocytic leukemia.

Blood

Waldmann TA,Conlon KC,Stewart DM,Worthy TA,Janik JE,Fleisher TA,Albert PS,Figg WD,Spencer SD,Raffeld M,Decker JR,Goldman CK,Bryant BR,Petrus MN,Creekmore SP,Morris JC

Thu Jan 17 00:00:00 UTC 2013

IL-7 and IL-15 instruct the generation of human memory stem T cells from naive precursors.

Blood

Cieri N,Camisa B,Cocchiarella F,Forcato M,Oliveira G,Provasi E,Bondanza A,Bordignon C,Peccatori J,Ciceri F,Lupo-Stanghellini MT,Mavilio F,Mondino A,Bicciato S,Recchia A,Bonini C

Thu Jan 24 00:00:00 UTC 2013

Natural killer cells promote immune tolerance by regulating inflammatory TH17 cells at the human maternal-fetal interface.

Proceedings of the National Academy of Sciences of the United States of America

Fu B,Li X,Sun R,Tong X,Ling B,Tian Z,Wei H

Tue Jan 15 00:00:00 UTC 2013

High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas.

Blood

Zocchi MR,Catellani S,Canevali P,Tavella S,Garuti A,Villaggio B,Zunino A,Gobbi M,Fraternali-Orcioni G,Kunkl A,Ravetti JL,Boero S,Musso A,Poggi A

Thu Feb 09 00:00:00 UTC 2012

Aurora kinase A-specific T-cell receptor gene transfer redirects T lymphocytes to display effective antileukemia reactivity.

Blood

Nagai K,Ochi T,Fujiwara H,An J,Shirakata T,Mineno J,Kuzushima K,Shiku H,Melenhorst JJ,Gostick E,Price DA,Ishii E,Yasukawa M

Thu Jan 12 00:00:00 UTC 2012

Antibody-dependent anti-cytomegalovirus activity of human γδ T cells expressing CD16 (FcγRIIIa).

Blood

Couzi L,Pitard V,Sicard X,Garrigue I,Hawchar O,Merville P,Moreau JF,Déchanet-Merville J

Thu Feb 09 00:00:00 UTC 2012

Dasatinib enhances the expansion of CD56+CD3- NK cells from cord blood.

Blood

Tanaka J,Sugita J,Shiratori S,Shigematsu A,Imamura M

Thu Jun 21 00:00:00 UTC 2012

K-RAS GTPase- and B-RAF kinase-mediated T-cell tolerance defects in rheumatoid arthritis.

Proceedings of the National Academy of Sciences of the United States of America

Singh K,Deshpande P,Li G,Yu M,Pryshchep S,Cavanagh M,Weyand CM,Goronzy JJ

Tue Jun 19 00:00:00 UTC 2012

TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.

PLoS pathogens

Sun C,Fu B,Gao Y,Liao X,Sun R,Tian Z,Wei H

Mon Jul 09 00:00:00 UTC 2012

IRX-2, a novel biologic, favors the expansion of T effector over T regulatory cells in a human tumor microenvironment model.

Journal of molecular medicine (Berlin, Germany)

Schilling B,Harasymczuk M,Schuler P,Egan JE,Whiteside TL

Wed Feb 01 00:00:00 UTC 2012

IL-21 can supplement suboptimal Lck-independent MAPK activation in a STAT-3-dependent manner in human CD8(+) T cells.

Journal of immunology (Baltimore, Md. : 1950)

Imataki O,Ansén S,Tanaka M,Butler MO,Berezovskaya A,Milstein MI,Kuzushima K,Nadler LM,Hirano N

Wed Feb 15 00:00:00 UTC 2012

CD8 T cell priming in the presence of IFN-α renders CTLs with improved responsiveness to homeostatic cytokines and recall antigens: important traits for adoptive T cell therapy.

Journal of immunology (Baltimore, Md. : 1950)

Hervas-Stubbs S,Mancheño U,Riezu-Boj JI,Larraga A,Ochoa MC,Alignani D,Alfaro C,Morales-Kastresana A,Gonzalez I,Larrea E,Pircher H,Le Bon A,Lopez-Picazo JM,Martín-Algarra S,Prieto J,Melero I

Mon Oct 01 00:00:00 UTC 2012

IL-2 upregulates CD86 expression on human CD4(+) and CD8(+) T cells.

Journal of immunology (Baltimore, Md. : 1950)

Paine A,Kirchner H,Immenschuh S,Oelke M,Blasczyk R,Eiz-Vesper B

Wed Feb 15 00:00:00 UTC 2012

Antiviral and regulatory T cell immunity in a patient with stromal interaction molecule 1 deficiency.

Journal of immunology (Baltimore, Md. : 1950)

Fuchs S,Rensing-Ehl A,Speckmann C,Bengsch B,Schmitt-Graeff A,Bondzio I,Maul-Pavicic A,Bass T,Vraetz T,Strahm B,Ankermann T,Benson M,Caliebe A,Fölster-Holst R,Kaiser P,Thimme R,Schamel WW,Schwarz K,Feske S,Ehl S

Wed Feb 01 00:00:00 UTC 2012

Fetal-specific CD8+ cytotoxic T cell responses develop during normal human pregnancy and exhibit broad functional capacity.

Journal of immunology (Baltimore, Md. : 1950)

Lissauer D,Piper K,Goodyear O,Kilby MD,Moss PA

Sun Jul 15 00:00:00 UTC 2012

Identification of circulating human antigen-reactive CD4+ FOXP3+ natural regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Litjens NH,Boer K,Betjes MG

Wed Feb 01 00:00:00 UTC 2012

T-cell receptor-optimized peptide skewing of the T-cell repertoire can enhance antigen targeting.

The Journal of biological chemistry

Ekeruche-Makinde J,Clement M,Cole DK,Edwards ES,Ladell K,Miles JJ,Matthews KK,Fuller A,Lloyd KA,Madura F,Dolton GM,Pentier J,Lissina A,Gostick E,Baxter TK,Baker BM,Rizkallah PJ,Price DA,Wooldridge L,Sewell AK

Fri Oct 26 00:00:00 UTC 2012

Signal transducer and activator of transcription 5b (Stat5b) serine 193 is a novel cytokine-induced phospho-regulatory site that is constitutively activated in primary hematopoietic malignancies.

The Journal of biological chemistry

Mitra A,Ross JA,Rodriguez G,Nagy ZS,Wilson HL,Kirken RA

Fri May 11 00:00:00 UTC 2012

Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.

PloS one

Nielsen N,Ødum N,Ursø B,Lanier LL,Spee P

Mon Aug 27 00:00:00 UTC 2012

MHC class I-presented T cell epitopes identified by immunoproteomics analysis are targets for a cross reactive influenza-specific T cell response.

PloS one

Testa JS,Shetty V,Hafner J,Nickens Z,Kamal S,Sinnathamby G,Philip R

Wed Apr 24 00:00:00 UTC 2013

Human natural killer cell maturation defect supports in vivo CD56(bright) to CD56(dim) lineage development.

PloS one

Domaica CI,Fuertes MB,Uriarte I,Girart MV,Sardañons J,Comas DI,Di Giovanni D,Gaillard MI,Bezrodnik L,Zwirner NW

Thu May 30 00:00:00 UTC 2013

MiRNA-mediated control of HLA-G expression and function.

PloS one

Manaster I,Goldman-Wohl D,Greenfield C,Nachmani D,Tsukerman P,Hamani Y,Yagel S,Mandelboim O

Mon Aug 13 00:00:00 UTC 2012

Regulation and gene expression profiling of NKG2D positive human cytomegalovirus-primed CD4+ T-cells.

PloS one

Jensen H,Folkersen L,Skov S

Tue Feb 19 00:00:00 UTC 2013

Generation of allo-restricted peptide-specific T cells using RNA-pulsed dendritic cells: A three phase experimental procedure.

Oncoimmunology

Wilde S,Geiger C,Milosevic S,Mosetter B,Eichenlaub S,Schendel DJ

Thu Mar 01 00:00:00 UTC 2012

A pilot study of IL-2Rα blockade during lymphopenia depletes regulatory T-cells and correlates with enhanced immunity in patients with glioblastoma.

PloS one

Sampson JH,Schmittling RJ,Archer GE,Congdon KL,Nair SK,Reap EA,Desjardins A,Friedman AH,Friedman HS,Herndon JE,Coan A,McLendon RE,Reardon DA,Vredenburgh JJ,Bigner DD,Mitchell DA

AF-200-15 was used in In vitro experiments to examine the combination of daclizumab and EGFRvIII targeted peptide vaccination for depleting regulatory T cells in glioblastoma treated with lymphodepleting temozolomide.

Mon Aug 27 00:00:00 UTC 2012

Activation of STAT6 by STING is critical for antiviral innate immunity.

Cell

Chen H,Sun H,You F,Sun W,Zhou X,Chen L,Yang J,Wang Y,Tang H,Guan Y,Xia W,Gu J,Ishikawa H,Gutman D,Barber G,Qin Z,Jiang Z

Fri Oct 14 00:00:00 UTC 2011

Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the γc cytokines and TGF-β1.

Blood

Park YP,Choi SC,Kiesler P,Gil-Krzewska A,Borrego F,Weck J,Krzewski K,Coligan JE

Thu Sep 15 00:00:00 UTC 2011

Severe impairment of IFN-γ and IFN-α responses in cells of a patient with a novel STAT1 splicing mutation.

Blood

Vairo D,Tassone L,Tabellini G,Tamassia N,Gasperini S,Bazzoni F,Plebani A,Porta F,Notarangelo LD,Parolini S,Giliani S,Badolato R

Thu Aug 18 00:00:00 UTC 2011

Control of Plasmodium falciparum erythrocytic cycle: γδ T cells target the red blood cell-invasive merozoites.

Blood

Costa G,Loizon S,Guenot M,Mocan I,Halary F,de Saint-Basile G,Pitard V,Déchanet-Merville J,Moreau JF,Troye-Blomberg M,Mercereau-Puijalon O,Behr C

Thu Dec 22 00:00:00 UTC 2011

Novel adoptive T-cell immunotherapy using a WT1-specific TCR vector encoding silencers for endogenous TCRs shows marked antileukemia reactivity and safety.

Blood

Ochi T,Fujiwara H,Okamoto S,An J,Nagai K,Shirakata T,Mineno J,Kuzushima K,Shiku H,Yasukawa M

Thu Aug 11 00:00:00 UTC 2011

Activation of Wnt signaling arrests effector differentiation in human peripheral and cord blood-derived T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Muralidharan S,Hanley PJ,Liu E,Chakraborty R,Bollard C,Shpall E,Rooney C,Savoldo B,Rodgers J,Dotti G

Tue Nov 15 00:00:00 UTC 2011

IL-7 promotes CD95-induced apoptosis in B cells via the IFN-γ/STAT1 pathway.

PloS one

Sammicheli S,Dang VP,Ruffin N,Pham HT,Lantto R,Vivar N,Chiodi F,Rethi B

Mon Aug 27 00:00:00 UTC 2012

KIR and HLA-C interactions promote differential dendritic cell maturation and is a major determinant of graft failure following kidney transplantation.

PloS one

Hanvesakul R,Kubal C,Moore J,Neil D,Cook M,Ball S,Briggs D,Moss P,Cockwell P

Fri Dec 23 00:00:00 UTC 2011

Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.

PloS one

Yu Y,Zitzner JR,Houlihan J,Herrera N,Xu L,Miller J,Mathew JM,Tambur AR,Luo X

Mon Aug 27 00:00:00 UTC 2012

Differentiation of chronic lymphocytic leukemia B cells into immunoglobulin secreting cells decreases LEF-1 expression.

PloS one

Gutierrez A,Arendt BK,Tschumper RC,Kay NE,Zent CS,Jelinek DF

Mon Feb 13 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: il 15; IL-15; ILN; Interleukin; Interleukin-15; Interleukin15; M-IL-15; MGC9721

Human IL-15, Animal-Free Recombinant Protein, PeproTech®

Human IL-15, Animal-Free Protein (AF-200-15-10UG) in Functional

Product Details

AF-200-15-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (MISC)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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