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Invitrogen
Human IL-5 Recombinant Protein
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Product Details
RIL510
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human IL-5 recombinant protein is a homodimer containing 116 amino acid subunits
Molecular weight
26 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE and HPLC
Activity
ED50 ≤ 0.15 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Use: IL-5 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0ºC. Cytokines diluted as indicated above should be divided into aliquots and stored at -70ºC. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell differentiation factor I; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: EDF; IL-5; IL5; TRF
UniProt ID: (Human) P05113
Entrez Gene ID: (Human) 3567
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