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Invitrogen
Human IkB beta (aa 193-257) Control Fragment Recombinant Protein
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Product Details
RP-105278
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (89%), Rat (89%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84615. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunits of the kinase and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I kappa B beta; I-kappa-B-beta; ikappaBbeta; IKB B; IKB beta; IkB-B; ikB-beta; IkBbeta; NF kappa BIB; NF-kappa-B inhibitor beta; NF-kappa-BIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; Thyroid receptor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP 9; TRIP-9
Gene Aliases: IKBB; NFKBIB; TRIP9
UniProt ID: (Human) Q15653
Entrez Gene ID: (Human) 4793
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