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Invitrogen
Human KCC2 (aa 938-1005) Control Fragment Recombinant Protein
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Product Details
RP-104242
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
ILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSC
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-64474. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; hKCC2; K-Cl cotransporter 2; KCC 2; Neuronal K-Cl cotransporter; S12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5
Gene Aliases: EIEE34; EIG14; hKCC2; KCC2; KIAA1176; SLC12A5
UniProt ID: (Human) Q9H2X9
Entrez Gene ID: (Human) 57468
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