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Invitrogen
Human KLHL29 (aa 773-848) Control Fragment Recombinant Protein
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Product Details
RP-109856
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
AVTLNGFVFILGGAYARATTIYDPEKGNIKAGPNMNHSRQFCSAVVLDGKIYATGGIVSSEGPALGNMEAYEPTTN
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-144759. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
KLHL29 (Kelch-like protein 29), also known as KBTBD9, is a 655 amino acid protein that is related to the Drosophila Kelch protein. Mutations affecting Kelch function result in failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL29 contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. KLHL29 exists as two alternatively spliced isoforms which are encoded by a gene that maps to human chromosome 2p24.1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: kelch repeat and BTB (POZ) domain containing 9; Kelch repeat and BTB domain-containing protein 9; kelch-like 29; kelch-like family member 29; Kelch-like protein 29
Gene Aliases: KBTBD9; KIAA1921; KLHL29
UniProt ID: (Human) Q96CT2
Entrez Gene ID: (Human) 114818
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