Invitrogen

Human Kallikrein 1 His-tag Recombinant Protein

Human Kallikrein 1 His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Human Kallikrein 1 His-tag Protein (RP-88086) in SDS-PAGE

SDS-PAGE analysis of Human Kallikrein 1 His-tag (Product # RP-88086) shows purity to be: ≥ 95 % (SDS-PAGE), ≥ 95 % (SEC-HPLC).

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Product Details

RP-88086

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human KLK1 precursor, amino acids Met1-Ser262 (Accession # NP_002248.1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

50 kDa

Class

Recombinant

Type

Protein

Purity

>95% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,500 pmoles/min/ µg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (1205 µL) be added to the vial to prepare a stock solution of 0.83 mg/mL.

Concentration is measured by UV.

Target Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: glandular kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein serine protease 1; Kallikrein-1; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein