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Invitrogen
Human LDB2 (aa 265-298) Control Fragment Recombinant Protein
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Product Details
RP-105455
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SSTSNSSAGNNANSTGSKKKTTAANLSLSSQVPD
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (79%), Rat (79%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-67002. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins. These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes. The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 and Clim2/Lbd1/Nli; and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carboxyl-terminal LIM domain-binding protein 1; CLIM-1; LDB-2; LIM binding domain 2; LIM domain-binding factor CLIM1; LIM domain-binding factor-2; LIM domain-binding protein 2
Gene Aliases: CLIM1; LDB-2; LDB1; LDB2
UniProt ID: (Human) O43679
Entrez Gene ID: (Human) 9079
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