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Invitrogen
Human MAGED4 (aa 2-117) Control Fragment Recombinant Protein
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Product Details
RP-102110
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
AEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAA
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (25%), Rat (25%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-51961. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAGE-D4 antigen; MAGE-E1 antigen; melanoma antigen family D, 4; melanoma antigen family D4; Melanoma-associated antigen D4
Gene Aliases: KIAA1859; MAGE-E1; MAGE1; MAGED4; MAGED4A; MAGED4B; MAGEE1
UniProt ID: (Human) Q96JG8
Entrez Gene ID: (Human) 728239
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