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Invitrogen
Human MAP4K5 Control Fragment Recombinant Protein
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Product Details
RP-109666
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
WLYVINNTLMSLSEGKTFQLYSHNLIALFEHAKKPGLAAHIQTHRFPDRI
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (98%), Rat (98%).
This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
MAP4K5 (KHS1) is a serine/threonine kinase that belongs to the GCK family of kinases and has been implicated as an upstream regulator of MAP kinase signaling pathways. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for MAP4K5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: germinal center kinase-related; KHS; Kinase homologous to SPS1/STE20; MAPK/ERK kinase kinase kinase 5; MEK kinase kinase 5; MEKKK 5; Mitogen-activated protein kinase kinase kinase kinase 5
Gene Aliases: GCKR; KHS; KHS1; MAP4K5; MAPKKKK5
UniProt ID: (Human) Q9Y4K4
Entrez Gene ID: (Human) 11183
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