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Invitrogen
Human MARCH10 (aa 379-475) Control Fragment Recombinant Protein
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Product Details
RP-93424
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
LPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVGVNAENVWSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPAS
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (32%), Rat (32%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-54763. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10-Mar; FLJ35757; MARCH-X; membrane associated ring finger 10; membrane-associated ring finger (C3HC4) 10, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; Probable E3 ubiquitin-protein ligase MARCHF10; RING finger protein 190; RING-type E3 ubiquitin transferase MARCHF10; testis secretory sperm-binding protein Li 228n
Gene Aliases: MARCH-X; MARCH10; MARCHF10; RNF190
UniProt ID: (Human) Q8NA82
Entrez Gene ID: (Human) 162333
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