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Invitrogen
Human MED25 (aa 621-674) Control Fragment Recombinant Protein
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Product Details
RP-105458
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
PPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPG
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (94%), Rat (94%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84792. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC/mediator transcriptional coactivator subunit; ARC92; DKFZp434K0512; Mediator complex subunit 25; Mediator of RNA polymerase II transcription subunit 25; mediator of RNA polymerase II transcription, subunit 25 homolog; MGC70671; p78; prostate-derived protein 78 kDa
Gene Aliases: ACID1; ARC92; BVSYS; CMT2B2; MED25; P78; PTOV2; TCBAP0758
UniProt ID: (Human) Q71SY5
Entrez Gene ID: (Human) 81857
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