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Invitrogen
Human MIER3 (aa 488-550) Control Fragment Recombinant Protein
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Product Details
RP-103615
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VPESFMNEVSVNNLGVDFENHTHHITSAKMAVSVADFGSLSANETNGFISAHALHQHAALHSE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (94%), Rat (94%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-64251. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
MIER3, known as Mesoderm Induction Early Response 1, Family Member 3, is a gene associated with epithelial-mesenchymal transition (EMT) processes particularly in cancer research. The gene is located on chromosome 7 and has been identified in studies as playing a crucial role in the progression of colorectal and breast cancers. Structurally, MIER3 is involved in chromatin remodeling and histone acetylation, functioning together with HDAC1/2 and Snail proteins. Its expression profile has been characterized using bioinformatics, real-time PCR, and Western blotting, indicating that MIER3 interacts closely with cellular mechanisms that modulate gene expression and cellular differentiation. The gene's role in cancer has been explored through its capacity to induce EMT, which affects tumor metastasis and cell migration, highlighting its potential as a therapeutic target in oncology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mesoderm induction early response 1, family member 3; Mesoderm induction early response protein 3; Mi-er3
Gene Aliases: MIER3
UniProt ID: (Human) Q7Z3K6
Entrez Gene ID: (Human) 166968
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