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Invitrogen
Human MRLC2 (aa 143-172) Control Fragment Recombinant Protein
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Product Details
RP-104383
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
YREAPIDKKGNFNYIEFTRILKHGAKDKDD
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83767. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
MRLC2 is involved in the the activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MLC-2; MLC-2A; MLC20; MLCB; MRLC3; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin, light chain 12B, regulatory; RLC; SHUJUN-1
Gene Aliases: MLC-B; MRLC2; MYL12B; MYLC2B
UniProt ID: (Human) O14950
Entrez Gene ID: (Human) 103910
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