Product References
Correction of F8 intron 1 inversion in hemophilia A patient-specific iPSCs by CRISPR/Cas9 mediated gene editing.
Frontiers in genetics
Hu Z,Wu Y,Xiao R,Zhao J,Chen Y,Wu L,Zhou M,Liang D
120-14P was used in In vitro experiments to rescue the 140kb reversion mutation by gene addition of a 975 bp fragment in the hemophilia A patient-derived induced pluripotent stem cell (HA-iPSCs) with intron 1 inversion (Inv1) mutation, providing a promising gene correction strategy for genetic disease with large sequence variants.
Tue Mar 28 00:00:00 UTC 2023
A desert lncRNA HIDEN regulates human endoderm differentiation via interacting with IMP1 and stabilizing FZD5 mRNA.
Genome biology
Lu P,Yang J,Li M,Wen S,Zhang T,Yan C,Liu R,Xiao Y,Wang X,Jiang W
120-14P was used in In vitro experiments to suggest that desert lncRNA HIDEN facilitates the interaction between IMP1 and FZD5 mRNA, stabilizing FZD5 mRNA which activates WNT signaling and promotes human definitive endoderm differentiation.
Mon Apr 24 00:00:00 UTC 2023
Epiblast-like stem cells established by Wnt/β-catenin signaling manifest distinct features of formative pluripotency and germline competence.
Cell reports
Luo Q,Pui HP,Chen J,Yu L,Jannig PR,Pei Y,Zhao L,Chen X,Petropoulos S,Ruas JL,Wu J,Deng Q
120-14P was used in Cell Culture to develop single-cell stage label transfer (scSTALT) to study the formative pluripotency continuum and reveal that EpiLSCs recapitulate a unique developmental period in vivo, filling the gap of the formative pluripotency continuum between other published formative stem cells.
Tue Jan 31 00:00:00 UTC 2023
Self-organized yolk sac-like organoids allow for scalable generation of multipotent hematopoietic progenitor cells from induced pluripotent stem cells.
Cell reports methods
Tamaoki N,Siebert S,Maeda T,Ha NH,Good ML,Huang Y,Vodnala SK,Haro-Mora JJ,Uchida N,Tisdale JF,Sweeney CL,Choi U,Brault J,Koontz S,Malech HL,Yamazaki Y,Isonaka R,Goldstein DS,Kimura M,Takebe T,Zou J,Stroncek DF,Robey PG,Kruhlak MJ,Restifo NP,Vizcardo R
120-14P was used in Cell Culture to demonstrate that organoid-induced HPCs can be differentiated into erythroid cells, macrophages, and T lymphocytes with current maturation protocols.
Mon Apr 24 00:00:00 UTC 2023
Protocol for capturing trophectoderm stem cells reflecting the blastocyst stage.
STAR protocols
Seong J,Rivron NC
120-14P was used in Cell Culture to captures trophectoderm stem cells with enhanced transcriptomic similarity to the blastocyst trophectoderm and self-renewal, reduced differentiation.
Thu Mar 16 00:00:00 UTC 2023
Influence of glycan structure on the colonization of Streptococcus pneumoniae on human respiratory epithelial cells.
Proceedings of the National Academy of Sciences of the United States of America
Chun YY,Tan KS,Yu L,Pang M,Wong MHM,Nakamoto R,Chua WZ,Huee-Ping Wong A,Lew ZZR,Ong HH,Chow VT,Tran T,Yun Wang D,Sham LT
120-14P was used in Cell Culture to reveal glycomotifs of surface polysaccharides that are likely to be important for colonization and survival in the human airway.
Tue Mar 28 00:00:00 UTC 2023
Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging.
Cell reports methods
LaLone V,Aizenshtadt A,Goertz J,Skottvoll FS,Mota MB,You J,Zhao X,Berg HE,Stokowiec J,Yu M,Schwendeman A,Scholz H,Wilson SR,Krauss S,Stevens MM
120-14P was used in In vitro experiments to develop an integrated bioanalytical methodology, qRamanomics, to qualify Raman spectral imaging as a tissue phantom calibrated tool for quantitative spatial chemotyping of major classes of biomolecules.
Mon Apr 24 00:00:00 UTC 2023
Parallel use of human stem cell lung and heart models provide insights for SARS-CoV-2 treatment.
Stem cell reports
Rudraraju R,Gartner MJ,Neil JA,Stout ES,Chen J,Needham EJ,See M,Mackenzie-Kludas C,Yang Lee LY,Wang M,Pointer H,Karavendzas K,Abu-Bonsrah D,Drew D,Yang Sun YB,Tan JP,Sun G,Salavaty A,Charitakis N,Nim HT,Currie PD,Tham WH,Porrello E,Polo JM,Humphrey SJ,Ramialison M,Elliott DA,Subbarao K
120-14P was used in In vitro experiments to provide new insights into rational drug combinations for effective treatment of a virus that affects multiple organ systems.
Tue Jun 13 00:00:00 UTC 2023
Epigenetic inheritance is gated by naïve pluripotency and Dppa2.
The EMBO journal
Carlini V,Policarpi C,Hackett JA
120-14P was used in Sample Preparation (FC/FACS) to outline the role of chromatin factor Dppa2, and naive pluripotent-state in dictating epigenetic inheritence within mammals.
Mon Apr 04 00:00:00 UTC 2022
SARS-CoV-2 infects an in vitro model of the human developing pancreas through endocytosis.
iScience
Szlachcic WJ,Dabrowska A,Milewska A,Ziojla N,Blaszczyk K,Barreto-Duran E,Sanak M,Surmiak M,Owczarek K,Grzanka D,Durzynska J,Pyrc K,Borowiak M
120-14P was used in In vitro experiments to determin that SARS-CoV-2 receptors ACE2, TMPRSS2, and NRP1 are expressed in precursors of insulin-producing pancreatic β-cells, rendering them permissive to SARS-CoV-2 infection.
Fri Jul 15 00:00:00 UTC 2022
Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.
Molecular biology of the cell
Buss G,Stratton MB,Milenkovic L,Stearns T
120-14P was used in In vitro experiments to find that the average nuclear area increases approximately eight-fold over differentiation, but most TGCs do not have more than four centrioles.
Tue Nov 01 00:00:00 UTC 2022
Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.
Methods in molecular biology (Clifton, N.J.)
Balbasi E,Sezginmert D,Alganatay C,Terzi Cizmecioglu N
120-14P was used in Cell Culture to provide protocols for the directed differentiation of mESCs toward these lineages in vitro.
Wed Jul 27 00:00:00 UTC 2022
Depletion of Demethylase KDM6 Enhances Early Neuroectoderm Commitment of Human PSCs.
Frontiers in cell and developmental biology
Meng Y,Zhang T,Zheng R,Ding S,Yang J,Liu R,Jiang Y,Jiang W
120-14P was used in In vitro experiments to use a system of directed neuroectoderm differentiation from human embryonic stem cells to show that that KDM6B, an enzyme responsible to erase H3K27me3, is the most upregulated enzyme of histone methylation during neuroectoderm differentiation by transcriptome analysis.
Wed Sep 29 00:00:00 UTC 2021
Quantitative analysis of signaling responses during mouse primordial germ cell specification.
Biology open
Morgani SM,Hadjantonakis AK
120-14P was used in Sample Preparation (FC/FACS) to investigate the signalling response of BMP, MAPK and WNT in primordial germ cells of mice.
Sat May 15 00:00:00 UTC 2021
SS18 regulates pluripotent-somatic transition through phase separation.
Nature communications
Kuang J,Zhai Z,Li P,Shi R,Guo W,Yao Y,Guo J,Zhao G,He J,Xu S,Wu C,Yu S,Zhou C,Wu L,Qin Y,Cai B,Li W,Wu Z,Li X,Chu S,Yang T,Wang B,Cao S,Li D,Zhang X,Chen J,Liu J,Pei D
Fri Jul 02 00:00:00 UTC 2021
Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells.
Cell reports
Kobayashi T,Castillo-Venzor A,Penfold CA,Morgan M,Mizuno N,Tang WWC,Osada Y,Hirao M,Yoshida F,Sato H,Nakauchi H,Hirabayashi M,Surani MA
Tue Oct 12 00:00:00 UTC 2021
Mitophagy mediates metabolic reprogramming of induced pluripotent stem cells undergoing endothelial differentiation.
The Journal of biological chemistry
Krantz S,Kim YM,Srivastava S,Leasure JW,Toth PT,Marsboom G,Rehman J
Wed Dec 01 00:00:00 UTC 2021
The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos.
eLife
Morgani SM,Su J,Nichols J,Massagué J,Hadjantonakis AK
120-14P was used in Cell Culture to determine that ras-responsive element-binding protein 1 is essential for mouse embryonic development, with a possible role in cancer and diseases involving loss of epithelial integrity.
Fri Apr 30 00:00:00 UTC 2021
CHD8 safeguards early neuroectoderm differentiation in human ESCs and protects from apoptosis during neurogenesis.
Cell death & disease
Ding S,Lan X,Meng Y,Yan C,Li M,Li X,Chen J,Jiang W
Fri Oct 22 00:00:00 UTC 2021
Rational design and optimization of synthetic gene switches for controlling cell-fate decisions in pluripotent stem cells.
Metabolic engineering
Haellman V,Saxena P,Jiang Y,Fussenegger M
120-14P was used in In vitro experiments to show that the VanA system can be multiplexed with the well-established reverse tetracycline-controlled transcriptional activator (rtTA) system to enable independent control of the expression of different transcription factors in human induced PSCs in order to enhance lineage specification towards early pancreatic progenitors.
Sat May 01 00:00:00 UTC 2021
Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation.
eLife
Cirnaru MD,Song S,Tshilenge KT,Corwin C,Mleczko J,Galicia Aguirre C,Benlhabib H,Bendl J,Apontes P,Fullard J,Creus-Muncunill J,Reyahi A,Nik AM,Carlsson P,Roussos P,Mooney SD,Ellerby LM,Ehrlich ME
120-14P was used in Cell Culture to dissect and manipulate the transcription factor networks regulating medium spiny neurone compartmentation and differentiation,.
Tue Oct 05 00:00:00 UTC 2021
GATA6-AS1 Regulates GATA6 Expression to Modulate Human Endoderm Differentiation.
Stem cell reports
Yang J,Lu P,Li M,Yan C,Zhang T,Jiang W
Tue Sep 08 00:00:00 UTC 2020
Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions.
Scientific reports
Regent F,Morizur L,Lesueur L,Habeler W,Plancheron A,Ben M'Barek K,Monville C
Tue Jul 23 00:00:00 UTC 2019
Delivery of pancreatic digestive enzymes into the gastrointestinal tract by pancreatic exocrine tissue transplant.
Scientific reports
Ito K,Matsuura K,Mihara Y,Sakamoto Y,Hasegawa K,Kokudo N,Shimizu T
Thu Apr 11 00:00:00 UTC 2019
Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture.
Scientific reports
Diekmann U,Wolling H,Dettmer R,Niwolik I,Naujok O,Buettner FFR
Wed Jan 30 00:00:00 UTC 2019
Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium.
Scientific reports
Suzuki K,Koyanagi-Aoi M,Uehara K,Hinata N,Fujisawa M,Aoi T
Fri Jul 19 00:00:00 UTC 2019
The Activin A-Peroxisome Proliferator-Activated Receptor Gamma Axis Contributes to the Transcriptome of GM-CSF-Conditioned Human Macrophages.
Frontiers in immunology
Nieto C,Bragado R,Municio C,Sierra-Filardi E,Alonso B,Escribese MM,Domínguez-Andrés J,Ardavín C,Castrillo A,Vega MA,Puig-Kröger A,Corbí AL
120-14P was used in In vitro experiments to investigate GM-CSF dependence on PPARγ.
Thu Feb 14 00:00:00 UTC 2019
Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.
Cell death discovery
Hoque A,Sivakumaran P,Bond ST,Ling NXY,Kong AM,Scott JW,Bandara N,Hernández D,Liu GS,Wong RCB,Ryan MT,Hausenloy DJ,Kemp BE,Oakhill JS,Drew BG,Pébay A,Lim SY
Sat Dec 01 00:00:00 UTC 2018
Interspecies Chimerism with Mammalian Pluripotent Stem Cells.
Cell
Wu J,Platero-Luengo A,Sakurai M,Sugawara A,Gil MA,Yamauchi T,Suzuki K,Bogliotti YS,Cuello C,Morales Valencia M,Okumura D,Luo J,Vilariño M,Parrilla I,Soto DA,Martinez CA,Hishida T,Sánchez-Bautista S,Martinez-Martinez ML,Wang H,Nohalez A,Aizawa E,Martinez-Redondo P,Ocampo A,Reddy P,Roca J,Maga EA,Esteban CR,Berggren WT,Nuñez Delicado E,Lajara J,Guillen I,Guillen P,Campistol JM,Martinez EA,Ross PJ,Izpisua Belmonte JC
Thu Jan 26 00:00:00 UTC 2017
The Expression of Human Cytomegalovirus MicroRNA MiR-UL148D during Latent Infection in Primary Myeloid Cells Inhibits Activin A-triggered Secretion of IL-6.
Scientific reports
Lau B,Poole E,Krishna B,Sellart I,Wills MR,Murphy E,Sinclair J
Fri Aug 05 00:00:00 UTC 2016
