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Invitrogen
Human N6AMT1 (aa 148-212) Control Fragment Recombinant Protein
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Product Details
RP-106922
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTTALSRQAGQETLSVLKFT
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (85%), Rat (85%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66242. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Heterodimeric methyltransferase that catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HemK methyltransferase family member 2; Lysine N-methyltransferase 9; M.HsaHemK2P; Methylarsonite methyltransferase N6AMT1; Methyltransferase N6AMT1; n(6)-adenine-specific DNA methyltransferase 1; N6-DNA-methyltransferase; Protein N(5)-glutamine methyltransferase
Gene Aliases: C21orf127; HEMK2; KMT9; m.HsaHemK2P; MTQ2; N6AMT; N6AMT1; PRED28
UniProt ID: (Human) Q9Y5N5
Entrez Gene ID: (Human) 29104
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