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Invitrogen
Human NADK (aa 136-222) Control Fragment Recombinant Protein
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Product Details
RP-106458
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQS
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65779. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The human trans-activation response (TAR) RNA binding protein 1 (TRBP1) was initially identified as a protein that binds to the HIV-1 TAR RNA and activates the long terminal repeat (LTR) expression in the absence and presence of the viral trans-activator Tat. This binding is enhanced by the presence of co-factors such as elongation factor 1-a (EF-1-a), polypyrimidine tract-binding protein (PTB), and the chaperonin-like protein stimulator of TAR RNA-binding proteins (SRB). TRBP1 may act to disengage RNA polymerase II from TAR during transcriptional elongation if the RNA polymerase stalls at the TAR during transcriptional elongation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ13052; NAD kinase; Poly(P)/ATP NAD kinase; ppnk
Gene Aliases: dJ283E3.1; NADK
UniProt ID: (Human) O95544
Entrez Gene ID: (Human) 65220
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