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Invitrogen
Human NFYC (aa 140-275) Control Fragment Recombinant Protein
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Product Details
RP-106594
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EPVQYYFTLAQQPTAVQVQGQQQGQQTTSSTTTIQPGQIIIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQGQAQQAQSGTGQTMQVMQQIITNTGEIQQIPVQLNAGQLQYIRLAQPVSGTQVVQGQIQTLATNAQ
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (99%), Rat (99%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65917. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold "handshake" motif in a manner analogous to the histone proteins, H2B and H2A, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAAT box DNA-binding protein subunit C; CCAAT binding factor subunit C; CCAAT transcription binding factor subunit gamma; DKFZp667G242; FLJ45775; histone H1 transcription factor large subunit 2A; NF-YC; Nuclear transcription factor Y subunit C; Nuclear transcription factor Y subunit gamma; nuclear transcription factor Y, gamma; transactivator HSM-1; Transactivator HSM-1/2; transcription factor NF-Y, C subunit
Gene Aliases: CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC; NFYC
UniProt ID: (Human) Q13952
Entrez Gene ID: (Human) 4802
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