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Invitrogen
Human NUP50 Control Fragment Recombinant Protein
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Product Details
RP-100841
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DSQQPSSSGLASSKACVGNAYHKQLAALDCSVRD
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (76%), Rat (76%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83860. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 50 kDa nucleoporin; CTA-268H5.7; DKFZP564A043; MGC39961; Nuclear pore complex protein Nup50; Nuclear pore-associated protein 60 kDa-like; nuclear pore-associated protein 60L; nucleoporin 50kD; nucleoporin 50kDa; Nucleoporin Nup50; NUP50 protein; OTTHUMP00000199530
Gene Aliases: NPAP60; NPAP60L; NUP50; PRO1146
UniProt ID: (Human) Q9UKX7
Entrez Gene ID: (Human) 10762
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