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Invitrogen
Human Opticin (aa 181-263) Control Fragment Recombinant Protein
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Product Details
RP-94534
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
RIDLSNNLISSIDNDAFRLLHALQDLILPENQLEALPVLPSGIEFLDVRLNRLQSSGIQPAAFRAMEKLQFLYLSDNLLDSIP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (84%), Rat (84%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57091. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Opticin, also known as OPTC or OPT, is a 332 amino acid protein that is secreted into the extracellular matrix and contains seven LRR repeats. Expressed in skin and fetal liver and also present in ocular tissues, such as the retina, cornea and iris, opticin functions to bind collagen fibrils and, via this interaction, is thought to regulate fibril spacing, morphology and organization. Opticin is subject to post-translational O-glycosylation and may be associated with the pathogenesis of primary open angle glaucoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Oculoglycan; Opticin
Gene Aliases: OPT; OPTC
UniProt ID: (Human) Q9UBM4
Entrez Gene ID: (Human) 26254
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