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Invitrogen
Human RASGRF1 (aa 755-855) Control Fragment Recombinant Protein
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Product Details
RP-98034
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
RKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (58%), Rat (58%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59803. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASPP1; GNRP; guanine nucleotide exchange factor; guanine nucleotide-releasing factor 1; guanine nucleotide-releasing factor, 55 kD; Guanine nucleotide-releasing protein; KIAA0771; PPP1R13B; Ras protein-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific guanine nucleotide-releasing factor, CDC25 homolog; Ras-specific nucleotide exchange factor CDC25
Gene Aliases: CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1; RASGRF1
UniProt ID: (Human) Q13972
Entrez Gene ID: (Human) 5923
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