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Invitrogen
Human RBM5 (aa 343-472) Control Fragment Recombinant Protein
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Product Details
RP-89627
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
WSSTQSQSGEGGSVDYSYLQPGQDGYAQYAQYSQDYQQFYQQQAGGLESDASSASGTAVTTTSAAVVSQSPQLYNQTSNPPGSPTEEAQPSTSTSTQAPAASPTGVVPGTKYAVPDTSTYQYDESSGYYY
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53586. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
DEF-3 and LUCA15 belong to an evolutionarily conserved family of RNA binding proteins and share similar expression patterns. Both DEF-3 and LUCA15 are highly expressed in adult heart and thymus as well as fetal kidney. Conversely, fetal thymus and adult kidney express very little DEF-3 and LUCA15. In the haemopoietic system of mice, the expression of DEF-3 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development. Both DEF-3 and LUCA15 contain two zinc finger motifs, a bipartite nuclear signal and two RNA binding motifs. DEF-3 and LUCA15 are capable of specifically binding poly(G) RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein G15; Putative tumor suppressor LUCA15; Renal carcinoma antigen NY-REN-9; RNA-binding motif protein 5; RNA-binding protein 5
Gene Aliases: G15; H37; LUCA15; RBM5; RMB5
UniProt ID: (Human) P52756
Entrez Gene ID: (Human) 10181
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