Invitrogen

Human RNF186 (aa 1-50) Control Fragment Recombinant Protein

Product Details

RP-102671

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCP

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (58%), Rat (58%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57315. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The RNF186 gene encodes the RING finger protein 186, a member of the RING-type E3 ubiquitin ligase family. RNF186 is involved in the ubiquitin-proteasome pathway, playing a crucial role in tagging target proteins for degradation, thus influencing protein turnover and cellular homeostasis. This process is essential for regulating various cellular functions, including signal transduction, apoptosis, and response to stress. RNF186 has been implicated in the unfolded protein response (UPR) within the endoplasmic reticulum, where it helps in managing protein folding and maintaining cellular homeostasis, particularly under conditions of endoplasmic reticulum stress. Alterations in RNF186 expression or function have been associated with disorders such as inflammatory bowel disease and ulcerative colitis, suggesting its role in gastrointestinal health and disease. Research into RNF186 focuses on its regulatory mechanisms within the ubiquitin-proteasome system and its potential as a therapeutic target for conditions associated with protein misfolding and cellular stress responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase RNF186; RING finger protein 186