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Invitrogen
Human RPUSD3 (aa 83-163) Control Fragment Recombinant Protein
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Product Details
RP-96384
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VVDRKGPLVTLNKPQGLPVTGKPGELTLFSVLPELSQSLGLREQELQVVRASGKESSGLVLLSSCPQTASRLQKYFTHARR
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (85%), Rat (85%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58001. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
RNA Pseudouridine Synthase D3 (RPUSD3) is a gene that encodes a pseudouridine synthase, an enzyme responsible for catalyzing the isomerization of uridine to pseudouridine in RNA molecules. This post-transcriptional modification is crucial for the stability and function of tRNAs and rRNAs. RPUSD3, along with other pseudouridine synthases such as PUS1, PUSL1, and TRUB2, is predicted to localize to mitochondria, indicating its role in mitochondrial RNA processing and maturation. The pseudouridylation activity provided by RPUSD3 is essential for the proper folding and functionality of mitochondrial RNAs, which are fundamental for the biogenesis of the mitochondrial electron transport chain and ATP synthase. While detailed structural insights into RPUSD3 are not yet fully characterized, its enzymatic function is critical in maintaining cellular energy metabolism and overall mitochondrial health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mitochondrial mRNA pseudouridine synthase RPUSD3; RNA pseudouridylate synthase domain-containing protein 3
Gene Aliases: RPUSD3
UniProt ID: (Human) Q6P087
Entrez Gene ID: (Human) 285367
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