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Invitrogen
Human RSF1 (aa 664-739) Control Fragment Recombinant Protein
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Product Details
RP-105074
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VDLETLKEDSEFTKVEMDNLDNAQTSGIEEPSETKGSMQKSKFKYKLVPEEETTASENTEITSERQKEGIKLTIRI
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (%), Rat (%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66088. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
RSF1 is required for assembly of regular nucleosome arrays by the RSF chromatin remodelling complex. Rsf1 facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappaB transcription activation. Rsf1 represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain. Rsf1 interacts with SMARCA5/SNF2H to form the RSF complex and also binds the HBV pX/HBx protein, which is required to activate transcription of the viral genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HBV pX associated protein-8; HBV pX-associated protein 8; hepatitis B virus x associated protein; Hepatitis B virus X-associated protein; p325 subunit of RSF chromatin-remodeling complex; Remodeling and spacing factor 1; Rsf-1
Gene Aliases: HBXAP; p325; RSF-1; RSF1; XAP8
UniProt ID: (Human) Q96T23
Entrez Gene ID: (Human) 51773
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