Invitrogen

Human SSU72 (aa 1-61) Control Fragment Recombinant Protein

Product Details

RP-107756

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

MPSSPLRVAVVCSSNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTY

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-67113. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

SSU72 is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. The human Ssu72 is located on Chromosome 1p36 and seems to represent a single gene. It was identified as a pRb binding factor and was also shown to interact with TFIIB and yeast PTA1. The ABs recognize a single band of approximately 30 Kda on immunoblots of various human cell lines but do not work well on mouse tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CTD phosphatase SSU72; FLJ13048; RNA polymerase II subunit A C-terminal domain phosphatase SSU72; RP5-832C2.2