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Invitrogen
Human TAF1A (aa 68-159) Control Fragment Recombinant Protein
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Product Details
RP-94292
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
HQWQQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMESFNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (87%), Rat (87%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84194. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, also known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RNA polymerase I-specific TBP-associated factor 48 kDa; SL1, 48kD subunit; TAFI48; TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kD; TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa; TATA box binding protein associated factor 1A; TATA box-binding protein-associated factor 1A; TATA box-binding protein-associated factor RNA polymerase I subunit A; TATA-box binding protein associated factor, RNA polymerase I, A; TBP-associated factor 1A; Transcription factor SL1; Transcription initiation factor SL1/TIF-IB subunit A
Gene Aliases: MGC:17061; RAFI48; SL1; TAF1A; TAFI48
UniProt ID: (Human) Q15573
Entrez Gene ID: (Human) 9015
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