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Invitrogen
Human TAF5L (aa 152-254) Control Fragment Recombinant Protein
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Product Details
RP-105441
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
NFKLRAFLDNKYVVRLQEDSYNYLIRYLQSDNNTALCKVLTLHIHLDVQPAKRTDYQLYASGSSSRSENNGLEPPDMPSPILQNEAALEVLQESIKRVKDGPP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66966. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
TAF5L (TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L), also known as PAF65B, is a 589 amino acid protein that localizes to the nucleus and contains six WD repeats. Existing as a component of the PCAF histone acetylase complex, TAF5L interacts with a number of TAFs and, via this interaction, plays a role in histone acetylation and transcription initiation. Defects in the gene encoding TAF5L, which is expressed as multiple alternatively spliced isoforms, are associated with the pathogenesis of type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PAF65-beta; PCAF associated factor 65 beta; PCAF-associated factor 65 beta; TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L; TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa; TAF5L; TATA box binding protein associated factor 5 like
Gene Aliases: PAF65B; TAF5L
UniProt ID: (Human) O75529
Entrez Gene ID: (Human) 27097
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