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Invitrogen
Human TRMT5 (aa 355-491) Control Fragment Recombinant Protein
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Product Details
RP-88992
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKR
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (75%), Rat (75%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-51488. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 in selected tRNAs using S-adenosyl methionine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: M1G-methyltransferase; TRM5 tRNA methyltransferase 5 homolog; TRMT5; tRNA (guanine(37)-N(1))-methyltransferase; tRNA (guanine-N(1)-)-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5 homolog; tRNA-N1G37 methyltransferase
Gene Aliases: COXPD26; KIAA1393; TRM5; TRMT5
UniProt ID: (Human) Q32P41
Entrez Gene ID: (Human) 57570
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