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Invitrogen
Human TTLL10 (aa 313-414) Control Fragment Recombinant Protein
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Product Details
RP-99825
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
KPTASNQGKGIFLLRNQEEVAALQAKTRSMEDDPIHHKTPFRGPQARVVQRYIQNPLLVDGRKFDVRSYLLIACTTPYMIFFGHGYARLTLSLYDPHSSDLG
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (82%), Rat (82%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60090. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The TTLL10 gene encodes a protein that functions as a polyglycylase, playing an important role in the modification of nucleosome assembly protein 1 (NAP1) both in vivo and in vitro. This enzymatic activity is dependent on the presence of ATP and is abolished in mutant forms where the glutamate in the active site is replaced by valine. TTLL10 specifically incorporates glycine into recombinant NAP1 at Glu residues 359 and 360. This modification process likely has implications for protein interactions and cellular processes involving NAP1. Additionally, TTLL10 can perform tubulin glycylation when co-expressed with TTLL8, suggesting a complex interplay between these enzymes in cellular microtubule dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Inactive polyglycylase TTLL10; tubulin tyrosine ligase-like family member 10; tubulin tyrosine ligase-like family, member 10; tubulin tyrosine ligase-like family, member 5; Tubulin--tyrosine ligase-like protein 10
Gene Aliases: TTLL10; TTLL5
UniProt ID: (Human) Q6ZVT0
Entrez Gene ID: (Human) 254173
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