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Invitrogen
Human TWISTNB (aa 250-329) Control Fragment Recombinant Protein
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Product Details
RP-92637
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DADDTPMEESALQNTNNANGIWEEEPKKKKKKKKHQEVQDQDPVFQGSDSSGYQSDHKKKKKKRKHSEEAEFTPPLKCSP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (65%), Rat (65%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53913. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
DNA-directed RNA polymerase I subunit RPA43 or Twist neighbor protein (Twistnb)belongs to the eukaryotic RPA43 RNA polymerase subunit family. It catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.Twistnb is the component of RNA polymerase I which synthesizes ribosomal RNA precursors. It is widely expressed in all fetal and adult tissues, with highest expression in fetal lung, liver, and kidney, and low expression in all adult tissues. Twist genes are essential for embryonic development and are conserved from jellyfish to human.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase I subunit F; DNA-directed RNA polymerase I subunit RPA43; Twist neighbor protein
Gene Aliases: POLR1F; TWISTNB
UniProt ID: (Human) Q3B726
Entrez Gene ID: (Human) 221830
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