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Invitrogen
Human Themis (aa 525-618) Control Fragment Recombinant Protein
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Product Details
RP-102595
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
PFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (69%), Rat (69%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-56739. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA325O24.3; bA325O24.4; FLJ40584; GRB2-associated protein; MGC163388; Protein THEMIS; signaling phosphoprotein specific for T cells; thymocyte expressed molecule involved in selection; thymocyte selection pathway associated; Thymocyte-expressed molecule involved in selection
Gene Aliases: C6orf190; C6orf207; GASP; SPOT; THEMIS; TSEPA
UniProt ID: (Human) Q8N1K5
Entrez Gene ID: (Human) 387357
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