Invitrogen

Human V-ATPase E2 (aa 98-118) Control Fragment Recombinant Protein

Product Details

RP-109451

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

AKLRLSRIVEDPEVYQGLLDK

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

1M urea/PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (76%), Rat (76%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-140273. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2; testis secretory sperm-binding protein Li 235P; V-ATPase subunit E 2; V-type proton ATPase subunit E 2; Vacuolar proton pump subunit E 2; vacuolar-type proton-translocating ATPase subunit E1