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          • Proteins & Peptides ›
          • VEGF-121 Proteins
          The PeproTech website has now moved to Thermofisher.com. All orders for PeproTech products must be placed on thermofisher.com or offline via phone, fax, email, or distributor partner. For any questions, please contact PeproTech.CustomerService@thermofisher.com

          Gibco

          Human VEGF-121 Recombinant Protein, PeproTech®

          31 References
          View all (2) VEGF-121 proteins
          Datasheet
          Tech Support
          Datasheet
          Tech Support

          Cite Human VEGF-121 Recombinant Protein, PeproTech®

          • Testing Data (1)
          Human VEGF-121 Protein in Functional Assay (Functional)
          Group 53 Created with Sketch.
          Human VEGF-121 Protein in Functional Assay (Functional)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Human VEGF-121 Protein (100-20A-10UG) in Functional

          Bioassay analysis of Human VEGF-121 Recombinant Protein, PeproTech® (Product # 100-20A-1MG). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Human VEGF-121 Protein in Functional Assay (Functional)

          Product Details

          100-20A-10UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 2 publications 2 publications

          Functional Assay (Functional)

          Assay-dependent
          -

          In vitro Assay (IV)

          -
          View 20 publications 20 publications

          Miscellaneous PubMed (MISC)

          -
          View 8 publications 8 publications
          Product Specifications

          Species

          Human

          Published species

          Chicken, Dog, Human, Mouse, Rabbit, Rat, Sheep

          Expression System

          E. coli

          Amino acid sequence

          APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R

          Molecular weight

          28.4 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          ≥ 98% by SDS-PAGE gel and HPLC analyses.

          Endotoxin concentration

          <1 EU/µg

          Activity

          Determined by its ability to stimulate the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED50 for this effect is 1.0-5.0 ng/ml.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Purification

          purified

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          Product Specific Information

          Recombinant Human VEGF 121 is a 28.4 kDa, disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.

          This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

          Target Information

          Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several variants, one being VEGF-120. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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