Invitrogen

Human WDR4 Control Fragment Recombinant Protein

Product Details

RP-91778

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

AGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAEKKSQENKGEDAPLDQGSGAILASTFSNSGSYFALTDDSKRLILFRTKPWQCLSVRTVAR

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (76%), Rat (76%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53600. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

Required for 7-methylguanosine modification of tRNA. This protein is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD). which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes. including cell cycle progression. signal transduction. apoptosis. and gene regulation. This protein is excluded as a candidate for a form of nonsyndromic deafness (DFNB10). but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternative splicing of this gene generates at least 3 transcript variants. two of which diverge at the 3' UTR and thus encode the same protein products.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hWH; Protein Wuho homolog; TRM82 tRNA methyltransferase 82 homolog; tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4; tRNA (guanine-N(7)-)-methyltransferase subunit WDR4; WD repeat-containing protein 4; WDR4