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Invitrogen
Human WNK3 (aa 582-675) Control Fragment Recombinant Protein
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Product Details
RP-108120
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKV
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (59%), Rat (59%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-67465. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
WNK3 is a serine/threonine kinase involved in signal transduction and cellular communication. Members of the 'with no lysine' kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PRKWNK 3; Protein kinase lysine-deficient 3; Protein kinase with no lysine 3; Serine/threonine-protein kinase WNK3; WNK 3
Gene Aliases: KIAA1566; PRKWNK3; WNK3
UniProt ID: (Human) Q9BYP7
Entrez Gene ID: (Human) 65267
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