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Invitrogen
Human ZNF197 (aa 253-325) Control Fragment Recombinant Protein
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Product Details
RP-101574
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VTSLEWETMTENEEVTSKPSSSQRADSHKGTSKRLQGSVPQVLDFEEECEWQVLASQWGNETDERADTVKKVS
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (27%), Rat (27%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84210. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
ZNF197 product belongs to the zinc finger protein superfamily, members of which are regulatory proteins characterized by nucleic acid-binding zinc finger domains. The encoded protein contains 20 tandemly arrayed C2H2-type zinc fingers, a Kruppel-associated box (KRAB) domain, and a SCAN box. This transcript turns over rapidly and contains 3' UTR AUUUA motifs, which are often a hallmark of rapid turnover. It is overexpressed in some thyroid papillary carcinomas. ZNF197 is located in a cluster of zinc finger genes at 3p21. Two alternatively spliced transcripts encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pVHL-associated KRAB domain-containing protein; VHL-associated KRAB-A domain-containing protein; zinc finger protein 166; Zinc finger protein 197; zinc finger protein 20; Zinc finger protein with KRAB and SCAN domains 9; ZnF20
Gene Aliases: D3S1363E; P18; VHLaK; ZKSCAN9; ZNF166; ZNF197; ZNF20; ZSCAN41
UniProt ID: (Human) O14709
Entrez Gene ID: (Human) 10168
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